Avian microsatellite loci amplified and genotyped in the turkey

Locus

TM

MgCl

Amplicon Size

Result

# Alleles

Informative meioses

Chicken chrm

ADL0107

54

1.5

181-183

Pi

2

208

7

ADL0142

58

1.5

214-226

Pi

3

346

6

ADL0149

60

1.5

236-238

Pi

2

158

17

ADL0180

56

4.5

171-189

Pn

4

NA

7

ADL0184

56

1.5

130-153

Pi

2

204

18

ADL0188

56

4.5

125-127

Pi

2

42

1

ADL0254

60

1.5

89-91

Pi

2

207

28

ADL0262

60

1.5

112-121

Pn

3

NA

23

ADL0263

54

1.5

130-160

Pi

5

354

14

ADL0266

54

4.5

101-109

Pi

2

335

4

ADL0272

58

1.5

168-178

Pi

2

148

10

ADL0289

54

1.5

74-78

Pn

2

NA

23

ADL0292

60

1.5

125-137

Pi

3

254

5

ADL0306

56

1.5

112-116

Pi

2

47

3

ADL0315*

54

1.5

250-254

Pi

2

214

7

ADL0317

60

1.5

152-156

Pi

2

51

4

ADL0320

60

1.5

114-122

Pi

3

252

6

ADL0350

54

1.5

91-93

Pn

2

NA

1

ADL0353

58

1.5

154-160

Pn

2

NA

1

ADL0377

54

1.5

143-145

Pi

2

301

6

BCL2

54

1.5

189-207

Pn

2

NA

2

GCT0055

54

1.5

190-196

Pn

3

NA

12

LEI0043

56

1.5

107-110

Pi

2

50

3

LEI0064

60

1.5

308-317

Pi

2

47

7

LEI0066

60

1.5

300-328

Pi

4

357

14

LEI0070*

60

1.5

174-182

Pi

3

41

2

LEI0075

58

1.5

241-243

Pn

2

NA

Z

LEI0095

58

1.5

240-248

Pn

2

NA

4

LEI0098

58

1.5

145-149

Pi

2

158

14

LEI0103

60

1.5

247-259

Pi

2

55

10

LEI0105

58

1.5

152-156

Pi

2

290

2

LEI0106

58

1.5

296-306

Pi

3

410

1

LEI0107

58

1.5

224-226

Pi

2

252

1

LEI0117

54

1.5

192-208

Pi

3

148

2

LEI0130

58

1.5

240-246

Pi

2

137

9

LEI0132

58

1.5

228-230

Pn

2

NA

6@

LEI0138

58

1.5

183-185

Pi

2

86

1

LEI0158

58

1.5

92-94

Pi

2

251

7

LEI0169

58

1.5

228-230

Pi

2

79

1

LE0I358

56

1.5

162-180

Pi

4

125

UN

MCW0019

54

1.5

102-108

Pi

4

155

1

MCW0031

54

1.5

246-285

Pn

2

NA

15

MCW0034*

56

1.5

100-102

Pi

3

265

2

MCW0035

56

1.5

218-220

Pi

2

308

10

MCW0036

54

1.5

162-170

Pi

3

355

1

MCW0075

58

1.5

182-190

Pi

2

84

24

MCW0080

54

1.5

285-288

Pi

2

256

15

MCW0090

54

4.5

85-87

Pn

2

NA

5

MCW0093

56

1.5

240-270

Pi

5

357

3

MCW0098

56

1.5

225-243

Pi

3

213

4

MCW0130

58

1.5

240-242

Pi

2

42

2@

MCW0135

56

1.5

116-122

Pi

2

310

9

MCW0141

58

1.5

96-99

Pi

2

226

3

MCW0160

56

1.5

200-213

Pi

2

103

8

MCW0167

56

1.5

120-122

Pi

2

159

4

MCW0212

54

1.5

160-162

Pi

2

261

3

MCW0230

56

1.5

275-277

Pi

2

41

11

MCW0234

56

1.5

275-285

Pi

3

259

2

MCW0239

54

4.5

140-142

Pi

2

192

2

MCW0242

56

1.5

132-133

Pi

2

170

4@

MCW0244

56

1.5

101-102

Pi

2

124

13

MCW0250

56

1.5

220-230

Pi

3

314

6

MCW0267

54

1.5

263-268

Pi

3

203

9

MCW0302

58

1.5

140-146

Pi

2

268

4

MCW0304

58

1.5

271-281

Pi

3

170

19@

MCW0323

50

4.5

116-119

Pi

2

255

15

MCW0351

58

1.5

143-159

Pi

2

322

8

ROS0313

58

1.5

220-222

Pi

2

155

1@

ROS0316

58

1.5

110-111

Pi

2

86

13

ROS0321

56

1.5

226-240

Pi

3

38

2

ROS0326

58

1.5

218-220

Pn

2

NA

2@

ROS0339

58

1.5

282-320

Pn

2

NA

1

ROS0341

58

1.5

145-156

Pn

2

NA

UN

ROS0346

58

1.5

216-224

Pi

2

126

1@

ROS0348

58

1.5

200-210

Pi

2

93

15

ROS0354

58

1.5

162-164

Pi

2

44

14@

ROS0356

58

1.5

174-178

Pi

2

208

1@

UMA2.032

52

1.5

147-149

Pi

2

255

2

UMA2.080

56

1.5

147-157

Pi

2

208

2

UMA2.123

54

1.5

166-228

Pi

4

113

2

UMA2.195

52

1.5

91-93

Pi

2

44

2

Quail

GUJ0008

60

1.5

165-166

Pn

2

NA

8@

GUJ0010

60

1.5

144-146

Pi

2

78

UN

GUJ0013

60

1.5

136-138

Pi

2

208

UN

GUJ0034

56

1.5

214-215

Pn

2

NA

UN

GUJ0041

56

1.5

107-111

Pn

2

NA

UN

GUJ0042

60

1.5

197-199

Pn

2

NA

13@

Turkey

RHT0003

54

1.5

215-217

Pi

2

278

2@

RHT0010

56

1.5

211-219

Pi

2

84

21@

RHT0011

58

1.5

135-157

Pi

4

129

1@

TUM02

58

1.5

160-162

Pi

2

79

15@

TUM06

58

1.5

142-144

Pi

2

85

15@

TUM11*

60

1.5

198-202

Pi

2

43

4@

TUM12

58

1.5

212-214

Pi

2

126

20@

TUM17*

58

1.5

185-191

Pn

2

NA

1@

TUM18*

58

1.5

252-270

Pi

3

208

2@

TUM20*

60

1.5

192-206

Pi

2

342

UN

TUM22

56

1.5

132-144

Pi

2

86

17@

TUM23

58

1.5

154-158

Pi

2

41

1@

TUM25*

56

1.5

176-186

Pi

3

163

1@

TUM32*

60

1.5

193-197

Pn

2

NA

2@

TUM48*

58

1.5

209-211

Pi

2

86

2@

TUM49*

58

1.5

181-183

Pi

2

256

11@

WT-38

60

1.5

116-122

Pn

2

NA

1@

WT-54

60

1.5

157-162

Pi

2

84

17@

WT-75

60

1.5

277-291

Pi

2

39

5@

WT-77

60

1.5

163-173

Pn

3

NA

1@

WT-83

60

1.5

181-183

Pi

2

289

5@

WT-90

60

1.5

252-258

Pi

2

340

UN

Number of alleles was determined by genotyping representatives of the P and F1 generation of the UMN/NTBF mapping panel .  Pi denotes loci that are polymorphic and informative, Pn denote polymorphic loci that were either non-informative, or that could not be reliably scored.  Modified from Reed et al., Cytogenetic and Genome Research, 102:331-339 (2003). * denote loci where primers were redesigned from Genbank sequences.  @ denotes chromosome assignmement based on BLAST search of chicken genome sequence. Chicken primers obtained from US Poultry Genome Coordinator

Quail primers:
  1. Kayang B.B., Inoue-Murayama M., Nomura A., Kimura, K., Takahasi, H., Mizutani, M., and Ito, S.  2000.  Fifty microsatellite markers for Japanese quail.  J. Hered. 91:502-505.
Turkey primers :
  1. Donoghue AM, Sonstegard TS, King LM, Smith EJ, Burt DW:  Turkey sperm mobility influences paternity in the context of competitive fertilization. Biology Reprod 61, 422–427 (1999).
  2. Huang, H.B., Song, Y.Q., Hsei, M., Zahorchak, R., Chiu, J., Teuscher, C., and Smith, E.J.  1999.  Development and characterization of genetic mapping resources for the turkey (Meleagris gallopavo).  J. Hered. 90:240-242.
  3. Latch, E. K., E.J. Smith, and O.E. Rhodes, Jr. 2002.  Isolation and characterization of microsatellite loci in wild and domestic turkeys (Meleagris gallopavo).  Mol. Ecol. Notes 2:176-178.